Nutrient Agar with 5% Sodium Chloride, pH 9.5–10.0 (LMG Medium 254)

[所属分类:培养基配方] [发布时间:2021-6-23] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Agar
with 5% Sodium Chloride, pH 9.5–10.0
(LMG Medium 254)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................55.0g
Agar ............................................................................................15.0g
Peptone......................................................................................... 5.0g
Yeast extract..................................................................................2.0g
Lab-Lemco beef extract................................................................1.0g
pH 9.5–10.0
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C. Adjust pH to 9.5–10.0 with
sterile Na 2 CO 3 solution. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Bacillus haloalkaliphilus.