Nutrient Agar with Nalidixic Acid (ATCC Medium 2089)

[所属分类:培养基配方] [发布时间:2021-6-23] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Agar with Nalidixic Acid
(ATCC Medium 2089)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Pancreatic digest of gelatin...........................................................5.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
Nalidixic acid solution.............................................................10.0mL
pH 6.8 ± 0.2 at 25°C
Nalidixic Acid Solution:
Composition per 10.0mL:
Nalidixic acid................................................................................0.1g
Preparation of Nalidixic Acid Solution: Add nalidixic acid to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except nalidixic acid
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat while stirring and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti-
cally add sterile nalidixic acid solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation of nalidixic acid-resistant bacteria.