Nutrient Agar with Streptomycin

[所属分类:培养基配方] [发布时间:2021-6-23] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Agar with Streptomycin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Peptone..........................................................................................5.0g
Beef extract...................................................................................3.0g
Streptomycin solution..............................................................10.0mL
pH 6.8 ± 0.2 at 25°C
Streptomycin Solution:
Composition per 10.0mL:
Streptomycin...............................................................................0.02g
Preparation of Streptomycin Solution: Add streptomycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except streptomycin
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile
streptomycin solution. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use:  For the cultivation and maintenance of Corynebacterium glutam-
icum and Corynebacterium herculis.