Nutrient Agar with 5% Urea

[所属分类:培养基配方] [发布时间:2021-6-23] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Agar with 5% Urea

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Pancreatic digest of gelatin...........................................................5.0g
Beef extract...................................................................................3.0g
Urea solution..........................................................................100.0mL
pH 6.8 ± 0.2 at 25°C
Urea Solution:
Composition per 100.0mL:
Urea.............................................................................................50.0g
Preparation of Urea Solution: Add urea to distilled/deionized wa-
ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except urea solution, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile
urea solution. Mix thoroughly. Pour into sterile Petri dishes or asepti-
cally distribute into sterile tubes.
Use: For the cultivation and maintenance of Aspergillus species.