Nutrient Broth with Bovine Serum

[所属分类:培养基配方] [发布时间:2021-6-24] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Broth with Bovine Serum

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................10.0g
Pancreatic digest of gelatin...........................................................5.0g
Beef extract...................................................................................3.0g
Bovine serum.........................................................................100.0mL
pH 6.9 ± 0.2 at 25°C
Preparation of Medium: Add components, except bovine serum,
to distilled/deionized water and bring volume to 900.0mL. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add sterile bovine serum. Mix thoroughly. Aseptical-
ly distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Pseudomonas anguil-
liseptica.