Nutrient Broth with Rifampicin

[所属分类:培养基配方] [发布时间:2021-6-24] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Broth with Rifampicin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .........................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Beef extract...................................................................................1.0g
Rifampicin solution .................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Rifampicin Solution:
Composition per 10.0mL:
Rifampicin...............................................................................10.0mg
Preparation of Rifampicin Solution: Add rifampicin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except rifampicin solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Adjust pH to 7.4. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to room temperature. Aseptically add 10.0mL of sterile ri-
fampicin solution. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use: For the cultivation of Agrobacterium tumefaciens.