Nutrient Broth Salts Medium

[所属分类:培养基配方] [发布时间:2021-6-24] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Broth Salts Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin...........................................................5.0g
Beef extract...................................................................................3.0g
NaCl..............................................................................................3.0g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 · 2H 2 O.................................................................................0.15g
MnSO 4 ·H 2 O................................................................................0.05g
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Bacillus subtilis.