Nutrient Soil Extract Agar (LMG Medium 30)

[所属分类:培养基配方] [发布时间:2021-6-24] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Soil Extract Agar
(LMG Medium 30)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Yeast extract..................................................................................2.0g
Peptone ........................................................................................ 5.0g
NaCl..............................................................................................5.0g
Lab-Lemco beef extract................................................................1.0g
Soil extract....................................................................................1.0L
pH 7.4 ± 0.2 at 25°C
Soil Extract:
Composition per liter:
Soil..............................................................................................1.0kg
CaCO 3 ...........................................................................................2.0g
Preparation of Soil Extract: Add 1.0kg soil to 1.0L tap water. Au-
toclave for 30 min at 15 psi pressure–121°C. Add 2.0g CaCO 3 . Filter
through Whatman filter paper. Bring volume to 1.0L with tap water.
Mix thoroughly.
Preparation of Medium: Add components to 1.0L soil extract.
Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into
sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of soil bacteria.