NZC Broth

[所属分类:培养基配方] [发布时间:2021-6-25] [发布人:网站管理员2] [阅读次数:] [返回]

NZC Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

N-Z Amine A™..........................................................................10.0g
NaCl..............................................................................................5.0g
MgCl 2 ·6H 2 O..................................................................................2.0g
Casamino acids solution ............................................................5.0mL
Casamino Acids Solution:
Composition per 10.0mL:
Casamino acids.............................................................................2.0g
Preparation of Casamino Acids Solution: Add casamino acids
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except casamino acids
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 5.0mL of
sterile casamino acids solution. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation of Escherichia coli.