O157:H7(+) Plating Medium

[所属分类:培养基配方] [发布时间:2021-6-25] [发布人:网站管理员2] [阅读次数:] [返回]

O157:H7(+) Plating Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Sorbitol .......................................................................................12.0g
Salicin.........................................................................................10.0g
Inositol........................................................................................10.0g
Peptone .......................................................................................10.0g
Adonitol........................................................................................8.0g
NaCl..............................................................................................5.0g
Tryptone........................................................................................5.0g
Proteose peptone...........................................................................3.0g
Bile salts No. 3............................................................................1.25g
Indoxyl-β- D -galactopyranoside ..................................................0.12g
5-Bromo-4-chloro-3-indoxyl-β- D -galactopyranoside.................0.12g
Phenol Red....................................................................................0.1g
Isopropyl-β- D -thiogalactopyranoside...........................................0.1g
Novobiocin solution...................................................................1.0mL
Tellurite solution........................................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BIO-
SYNTH International, Inc.
Novobiocin Solution:
Composition per 10.0mL:
Novobiocin....................................................................................0.1g
Preparation of Novobiocin Solution: Add novobiocin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Tellurite Solution:
Composition per 10.0mL:
K 2 TeO 3 ........................................................................................0.01g
Preparation of Tellurite Solution: Add K 2 TeO 3 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Caution: Potassium tellurite is toxic.
Preparation of Medium: Add components, except novobiocin so-
lution and tellurite solution, to distilled/deionized water and bring vol-
ume to 998.0mL. Mix thoroughly. Gently heat while stirring and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Aseptially add 1.0mL sterile novobiocin solution and 1.0 sterile
tellurite solution. Mix thouroughly. Pour into sterile Petri dishes.
Use : For the detection of Escherichia coli O157:H7. E.coli O157:H7
grow with blue-black colonies and E.coli non-O157 with green-yellow
colonies.