Oat Flake Medium (DSMZ Medium 189)

[所属分类:培养基配方] [发布时间:2021-6-25] [发布人:网站管理员2] [阅读次数:] [返回]

Oat Flake Medium
(DSMZ Medium 189)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Oat flakes....................................................................................30.0g
Agar............................................................................................15.0g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add rolled oats to 500.0mL distilled/de-
ionized water. Gently heat and bring to boiling. Boil for 10 min. Filter.
Add agar and bring volume to 1.0L with distilled/deionized water. Mix
thoroughly. Gently heat and bring to boiling. Mix by shaking. Distrib-
ute into tubes or flasks. Autoclave for 20 min at 15 psi pressure–121°C.
Mix by shaking. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of actinomycetes and fungi.