Oatmeal Agar A (ISP-3) (DSMZ Medium 609)

[所属分类:培养基配方] [发布时间:2021-6-25] [发布人:网站管理员2] [阅读次数:] [返回]

Oatmeal Agar A
(ISP-3)
(DSMZ Medium 609)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Oatmeal (Quaker White Oats) ....................................................20.0g
Agar ............................................................................................18.0g
Trace salts solution.....................................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Trace Salts Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O..................................................................................0.1g
MnCl 2 ·4H 2 O..................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
Preparation of Trace Salts Solution:  Add components to
100.0mL distilled/deionized water. Mix thoroughly.
Preparation of Medium:  Add oats to 1.0L distilled/deionized wa-
ter. Adjust pH to 7.2. Gently heat and bring to boiling. Cook or steam
for 20 min. Filter through cheesecloth. Add 18.0g agar. Add 1.0mL
trace salts solution. Bring volume to 1.0L with distilled/deionized wa-
ter. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into Petri dishes or distribute to sterile tubes.
Use: For the cultivation of various fungi.