Ochromonas Medium

[所属分类:培养基配方] [发布时间:2021-6-25] [发布人:网站管理员2] [阅读次数:] [返回]

Ochromonas Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................20.0g
Vitamin-free casamino acids.......................................................10.0g
Diammonium hydrogen citrate.....................................................1.6g
KH 2 PO 4 .........................................................................................0.6g
DL -Methionine ..............................................................................0.4g
MgSO 4 ·7H 2 O................................................................................0.4g
CaCO 3 ...........................................................................................0.3g
ZnSO 4 ·7H 2 O...............................................................................0.22g
L -Cystine.......................................................................................0.2g
DL -Tryptophan ..............................................................................0.2g
MnSO 4 ·H 2 O..............................................................................0.123g
EDTA............................................................................................0.1g
Na 2 MoO 4 ·2H 2 O ............................................................................0.1g
FeSO 4 ·7H 2 O.............................................................................20.0mg
Inositol.....................................................................................20.0mg
CoSO 4 ·7H 2 O..............................................................................6.0mg
Choline chloride.........................................................................4.0mg
Thiamine....................................................................................4.0mg
PAB............................................................................................2.0mg
H 3 BO 3 ........................................................................................1.2mg
CuSO 4 ·5H 2 O...........................................................................800.0μg
Biotin ........................................................................................20.0μg
KI..............................................................................................20.0μg
Tween™ 80................................................................................2.0mL
pH 5.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.8.
Gently heat and bring to boiling. Continue boiling for 2–3 min. Distrib-
ute 5.0mL volumes into 6 × 125mm screw-capped test tubes. Swirl the
medium while dispensing to evenly distribute the fine precipitate
which forms. Autoclave for 10 min at 15 psi pressure–121°C. Do not
overheat.
Use: For the cultivation of Ochromonas malhamensis.