Oddoux Medium

[所属分类:培养基配方] [发布时间:2021-6-25] [发布人:网站管理员2] [阅读次数:] [返回]

Oddoux Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................13.0g
Malt extract...................................................................................8.0g
Glucose.........................................................................................7.0g
Casein hydrolysate........................................................................1.0g
Asparagine....................................................................................0.5g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.5g
Vitamin solution.......................................................................10.0mL
Vitamin Solution:
Composition per 10.0mL:
Calcium pantothenate ................................................................0.5mg
Nicotinamide..............................................................................0.5mg
Pyridoxine HCl..........................................................................0.5mg
Riboflavin..................................................................................0.5mg
Thiamine....................................................................................0.5mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Aseptically add 10.0mL of sterile vitamin so-
lution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation and maintenance of Boletus granulatus, Bol-
etus luteus, Lactarius deliciosus, and Tricholoma equestre.