ONR7a Medium (DSMZ Medium 983)

[所属分类:培养基配方] [发布时间:2021-6-28] [发布人:网站管理员2] [阅读次数:] [返回]

ONR7a Medium
(DSMZ Medium 983)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl .........................................................................................22.79g
Agar ............................................................................................15.0g
Na 2 SO 4 .......................................................................................3.98g
CaCl 2 ·2H 2 O.................................................................................1.46g
TAPSO .........................................................................................1.3g
MgCl 2 ·6H 2 O................................................................................1.18g
KCl .............................................................................................0.72g
NH 4 Cl .........................................................................................0.27g
Na 2 HPO 4 ..................................................................................89.0mg
NaBr ........................................................................................83.0mg
NaHCO 3 ...................................................................................31.0mg
H 3 BO 3 ......................................................................................27.0mg
SrCl 2 ·6H 2 O ..............................................................................24.0mg
NaF ............................................................................................2.6mg
FeCl 2 ·4H 2 O................................................................................2.0mg
Tetradecane..............................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except tetradecane, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 7.0. Distribute into tubes or flasks. Gently heat while stir-
ring and bring to boiling. Mix thoroughly. Autoclave for 15 min at 15
psi pressure–121°C. Pour into Petri dishes. Invert after agar solidifies.
Add tetradecane to the lid of the Petri dish.
Use: For the cultivation of Oleispira antarctica.