Organic Acid Medium KP (Organic Acid Medium, Kauffmann and Petersen)

[所属分类:培养基配方] [发布时间:2021-6-28] [发布人:网站管理员2] [阅读次数:] [返回]

Organic Acid Medium KP
(Organic Acid Medium, Kauffmann and Petersen)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Gelatin.........................................................................................10.0g
Bromthymol Blue .....................................................................0.024g
Organic acid solution.............................................................100.0mL
pH 7.4 ± 0.2 at 25°C
Organic Acid Solution:
Composition per 100.0mL:
Organic acid................................................................................10.0g
Preparation of Organic Acid Solution:  Add organic acid to dis-
tilled/deionized water and bring volume to 100.0mL. Sodium potassi-
um  D- tartrate, sodium citrate, or mucic acid may be used. Mix
thoroughly.
Preparation of Medium: Add components, except organic acid so-
lution, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile organic
acid solution. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation and differentiation of members of the Enter-
obacteriaceae based on their ability to utilize different organic acids as
carbon source. Bacteria that utilize tartrate, citrate, or mucate turn the
medium yellow.