Phenol Red Agar

[所属分类:培养基配方] [发布时间:2021-7-6] [发布人:网站管理员2] [阅读次数:] [返回]

Phenol Red Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
NaCl..............................................................................................5.0g
Phenol Red................................................................................0.018g
Carbohydrate solution..............................................................20.0mL
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Carbohydrate Solution:
Composition per 20.0mL:
Carbohydrate........................................................................5.0–10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 20.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 980.0mL.
Mix thoroughly. Adjust pH to 7.4 if necessary. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 20.0mL of
sterile carbohydrate solution. Pour into sterile Petri dishes or distribute
into sterile tubes. Allow tubes to cool in a slanted position.
Use: For the determination of fermentation reactions. Bacteria that can
ferment the added carbohydrate turn the medium yellow.