Phenol Red Carbohydrate Broth with Sodium Chloride (BAM M121)

[所属分类:培养基配方] [发布时间:2021-7-6] [发布人:网站管理员2] [阅读次数:] [返回]

Phenol Red Carbohydrate Broth
with Sodium Chloride
(BAM M121)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................30.0g
Pancreatic digest of casein..........................................................10.0g
Beef extract...................................................................................1.0g
Carbohydrate solution............................................................200.0mL
Phenol Red solution...................................................................7.2mL
pH 7.4 ± 0.2 at 25°C
Source:  This medium, without added NaCl, is available as a premixed
powder from BD Diagnostics.
Phenol Red Solution:
Composition per 10.0mL:
Phenol Red................................................................................0.025g
Preparation of Phenol Red Solution: Add Phenol Red to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Carbohydrate Solution:
Composition per 200.0mL:
Carbohydrate........................................................................5.0–10.0g
Preparation of Carbohydrate Solution: Add carbohydrate (5.0g
dulcitol, 10.0g lactose, or 10.0g sucrose) to distilled/deionized water
and bring volume to 200.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate so-
lution, to distilled/deionized water and bring volume to 800.0mL. Mix
thoroughly. Adjust pH to 7.4 if necessary. Distribute into tubes contain-
ing an inverted Durham tube. Fill each tube with 8.0mL of medium. Au-
toclave for 15 min at 13 psi pressure–118°C. Cool to 45°–50°C.
Aseptically add 2.0mL of sterile carbohydrate solution to each tube.
Use: For the determination of fermentation reactions in the differenti-
ation of halophilic Vibrio spp. Fermentation is determined by the pro-
duction of acid—broth turns yellow—and formation of gas—bubble
trapped in Durham tube.