Phenol Red Glucose Broth

[所属分类:培养基配方] [发布时间:2021-7-6] [发布人:网站管理员2] [阅读次数:] [返回]

Phenol Red Glucose Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................10.0g
Glucose .........................................................................................5.0g
NaCl..............................................................................................5.0g
Phenol Red................................................................................0.018g
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3 if
necessary. Distribute into tubes containing an inverted Durham tube.
Fill each tube with 10.0mL of medium. Autoclave for 15 min at 13 psi
pressure–118°C.
Use:  For determination of the ability of a microorganism to ferment
glucose. Fermentation is determined by the production of acid—broth
turns yellow—and formation of gas—bubble trapped in Durham tube.