Phenol Red Tartrate Agar

[所属分类:培养基配方] [发布时间:2021-7-7] [发布人:网站管理员2] [阅读次数:] [返回]

Phenol Red Tartrate Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Peptone........................................................................................10.0g
Potassium tartrate........................................................................10.0g
NaCl..............................................................................................5.0g
Phenol Red................................................................................0.024g
pH 7.6 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to cold distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and
bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min
at 13 psi pressure–118°C. Pour into sterile Petri dishes or leave in
tubes. Allow tubes to cool in an upright position.
Use: For the differentiation of Gram-negative bacteria of the intestinal
groups, particularly members of the Salmonella (paratyphoid) group
based on their ability to ferment tartrate.