Phenylalanine Agar (Phenylalanine Deaminase Medium)

[所属分类:培养基配方] [发布时间:2021-7-7] [发布人:网站管理员2] [阅读次数:] [返回]

Phenylalanine Agar
(Phenylalanine Deaminase Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................12.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................3.0g
DL- Phenylalanine...........................................................................2.0g
Na 2 HPO 4 .......................................................................................1.0g
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Distribute into tubes or flasks. Autoclave
for 10 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or
leave in tubes.
Use: For the differentiation of enteric Gram-negative bacilli on the
basis of their ability to produce phenylpyruvic acid from phenylala-
nine. After appropriate incubation of bacteria, ferric chloride reagent is
added on the agar. Formation of a green color in 1–5 min indicates the
production of phenylpyruvic acid.