Phenylethanol Blood Agar

[所属分类:培养基配方] [发布时间:2021-7-7] [发布人:网站管理员2] [阅读次数:] [返回]

Phenylethanol Blood Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Tryptose ......................................................................................10.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
Phenylethanol ...............................................................................2.5g
Blood, defibrinated..................................................................50.0mL
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except blood, to dis-
tilled/deionized water and bring volume to 950.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 13 psi pres-
sure–118°C. Cool to 45°–50°C. Aseptically add sterile defibrinated
blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the isolation of staphylococci and streptococci from speci-
mens containing a mixed flora.