Phenylobacterium Medium

[所属分类:培养基配方] [发布时间:2021-7-7] [发布人:网站管理员2] [阅读次数:] [返回]

Phenylobacterium Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Antipyrin.......................................................................................1.0g
Na 2 HPO 4 ·12H 2 O...........................................................................0.7g
(NH 4 ) 2 HPO 4 ..................................................................................0.7g
KH 2 PO 4 .........................................................................................0.3g
NH 4 H 2 PO 4 ....................................................................................0.3g
MgSO 4 ·7H 2 O..............................................................................0.25g
FeCl 3 ·6H 2 O...................................................................................0.2g
(NH 4 ) 2 SO 4 .....................................................................................0.1g
CaCl 2 ·6H 2 O..............................................................................50.0mg
H 3 BO 3 ........................................................................................0.5mg
MnSO 4 ·4H 2 O.............................................................................0.4mg
ZnSO 4 ·7H 2 O..............................................................................0.4mg
(NH 4 ) 2 MoO 4 ..............................................................................0.2mg
Biotin .........................................................................................0.1mg
CuSO 4 ·5H 2 O..............................................................................0.1mg
KI...............................................................................................0.1mg
Vitamin B 12 ...............................................................................30.0μg
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Phenylobacterium immobile.