Phosphate Mineral Salts Medium with Octane

[所属分类:培养基配方] [发布时间:2021-7-7] [发布人:网站管理员2] [阅读次数:] [返回]

Phosphate Mineral Salts Medium with Octane

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 HPO 4 ................................................................................10.0g
K 2 HPO 4 .........................................................................................5.0g
Na 2 SO 4 ..........................................................................................0.5g
Octane......................................................................................10.0mL
Preparation of Medium: Add components, except octane, to tap
water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Prior to inoculation, filter sterilize oc-
tane. Aseptically add sterile octane to sterile medium. Aseptically dis-
tribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Pseudomonas oleo-
vorans.