Picrophilus Medium

[所属分类:培养基配方] [发布时间:2021-7-8] [发布人:网站管理员2] [阅读次数:] [返回]

Picrophilus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1020.0mL:

(NH 4 ) 2 SO 4 ....................................................................................1.3g
KH 2 PO 4 .......................................................................................0.28g
MgSO 4 ·7H 2 O..............................................................................0.25g
CaCl 2 ·2H 2 O ................................................................................0.07g
FeCl 3 ·6H 2 O.................................................................................0.02g
Na 2 B 4 ·10H 2 O.............................................................................4.5mg
MnCl 2 ·4H 2 O ..............................................................................1.8mg
ZnSO 4 ·7H 2 O............................................................................0.22mg
CuCl 2 ·2H 2 O.............................................................................0.05mg
Na 2 MoO 4 ·2H 2 O.......................................................................0.03mg
VOSO 4 ·2H 2 O...........................................................................0.03mg
CoSO 4 ......................................................................................0.01mg
Sulfuric acid solution....................................................................1.0L
Yeast extract solution...............................................................20.0mL
pH 1.0 ± 0.2 at 25°C
Sulfuric Acid Solution:
Composition per liter:
H 2 SO 4 (0.5M solution)...........................................................300.0mL
Preparation of Sulfuric Acid Solution:  Add 300.0mL of 0.5M
H 2 SO 4 solution to 700.0mL of distilled/deionized water. Mix thor-
oughly.
Yeast Extract Solution:
Composition per 20.0mL:
Yeast extract..................................................................................2.0g
Preparation of Yeast Extract Solution:  Add yeast extract to dis-
tilled/deionized water and bring volume to 20.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Combine components, except yeast ex-
tract solution. Mix thoroughly. Autoclave for 15 min at 15 psi pres-
sure–121°C. Aseptically add 20.0mL of sterile yeast extract solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Picrophilus oshimae and Picrophilus torri-
dus.