Pike Streptococcal HiVeg Broth Base with Blood

[所属分类:培养基配方] [发布时间:2021-7-8] [发布人:网站管理员2] [阅读次数:] [返回]

Pike Streptococcal HiVeg Broth Base with Blood

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................10.0g
Plant hydrolysate.........................................................................10.0g
Plant hydrolysate No. 1...............................................................10.0g
Glucose .........................................................................................0.2g
NaN 3 .........................................................................................0.065g
Crystal Violet.............................................................................2.0mg
Rabbit blood, defibrinated .......................................................50.0mL
pH 7.4 ± 0.2 at 25°C
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components, except rabbit blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Distribute into flasks in 100.0mL
volumes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 5.0mL of sterile rabbit blood to each flask. Mix
thoroughly.
Use: For the isolation and enrichment of hemolytic streptococci from
throat swabs and other clinical specimens. After incubation of bacteria
for 18–24 hr in this medium, they may be isolated by streaking the cul-
ture onto blood agar plates.