Plant Mycoplasma Agar

[所属分类:培养基配方] [发布时间:2021-7-8] [发布人:网站管理员2] [阅读次数:] [返回]

Plant Mycoplasma Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 291.2mL:

Schneider’s Drosophila medium ...........................................160.0mL
Solution 1.................................................................................70.0mL
Fetal calf serum........................................................................50.0mL
Fresh yeast extract solution .....................................................10.0mL
Phenol Red (0.5% solution).......................................................1.2mL
pH 7.4 ± 0.2 at 25°C
Schneider’s Drosophila Medium:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.7g
NaCl..............................................................................................2.1g
Yeast extract..................................................................................2.0g
Trehalose.......................................................................................2.0g
D -Glucose......................................................................................2.0g
L- Glutamine ..................................................................................1.8g
L- Lysine·HCl.................................................................................1.7g
L- Proline........................................................................................1.7g
KCl................................................................................................1.6g
Na 2 HPO 4 ·7H 2 O.............................................................................1.3g
L- Glutamic acid.............................................................................0.8g
L- Methionine.................................................................................0.8g
CaCl 2 , anhydrous..........................................................................0.6g
KH 2 PO 4 .........................................................................................0.5g
β-Alanine......................................................................................0.5g
L- Tyrosine .....................................................................................0.5g
L- Arginine.....................................................................................0.4g
L- Aspartic acid..............................................................................0.4g
L- Histidine.....................................................................................0.4g
L- Threonine...................................................................................0.4g
NaHCO 3 ........................................................................................0.4g
Glycine..........................................................................................0.3g
L- Serine.........................................................................................0.3g
L- Valine.........................................................................................0.3g
L- Isoleucine...................................................................................0.2g
L- Leucine ......................................................................................0.2g
L- Phenylalanine.............................................................................0.2g
α-Ketoglutaric acid.......................................................................0.2g
Fumaric acid.................................................................................0.1g
Malic acid.....................................................................................0.1g
Succinic acid.................................................................................0.1g
L- Cystine.......................................................................................0.1g
L- Tryptophan.................................................................................0.1g
L- Cysteine ...................................................................................0.06g
Preparation of Schneider’s Drosophila Medium: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Filter sterilize.
Solution 1:
Composition per 70.0mL:
Sorbitol .........................................................................................7.0g
Noble agar.....................................................................................5.0g
Beef heart, solids from infusion....................................................5.0g
Peptone .........................................................................................1.8g
Pancreatic digest of casein............................................................1.0g
Sucrose..........................................................................................1.0g
NaCl..............................................................................................0.5g
D -Fructose.....................................................................................0.1g
D -Glucose......................................................................................0.1g
Preparation of Solution 1: Add components to distilled/deionized
water and bring volume to 70.0mL. Mix thoroughly. Adjust pH to 7.8
with 1N NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°C.
Fresh Yeast Extract  Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90
min at 15 psi pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6.6–6.8.
Preparation of Medium:  Bring fetal calf serum and Phenol Red so-
lution to 56°C. Rapidly bring Schneider’s Drosophila medium to 37°C.
Rapidly combine the components. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Spiroplasma floricola, Spiro-
plasma kunkelii, Spiroplasma melliferum, and Spiroplasma species.