Poly-β-Hydroxybutyrate Medium (PHB Medium)

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

Poly-β-Hydroxybutyrate Medium
(PHB Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Part A.....................................................................................900.0mL
Part B .....................................................................................100.0mL
pH 7.2 ± 0.2 at 25°C
Part A:
Composition per 900.0mL:
K 2 HPO 4 ·3H 2 O...............................................................................0.6g
KH 2 PO 4 .........................................................................................0.2g
MgSO 4 ·7H 2 O................................................................................0.2g
(NH 4 ) 2 SO 4 .....................................................................................0.2g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to 7.2.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Part B:
Composition per 100.0mL:
Glucose .......................................................................................10.0g
Preparation of Medium: Add glucose to distilled/deionized water
and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Aseptically combine 900.0mL of cooled,
sterile part A and 100.0mL of cooled, sterile part B. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and differentiation of Pseudomonas species
based on their ability to produce intracellular poly-β-hydroxybutyrate.
Production of poly-β-hydroxybutyrate is determined by staining cells
with Sudan Black B.