Porphyrobacter tepidarius Medium (DSMZ Medium 791)

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

Porphyrobacter tepidarius Medium
(DSMZ Medium 791)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium glutamate.........................................................................0.5g
Sodium succinate..........................................................................0.5g
Sodium acetate..............................................................................0.5g
Yeast extract..................................................................................0.5g
Casamino acids.............................................................................0.5g
(NH 4 ) 2 SO 4 ....................................................................................0.5g
Sodium thiosulfate........................................................................0.5g
Basal salts solution ....................................................................5.0mL
Phosphate solution.....................................................................5.0mL
Vitamin solution.........................................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Basal Salts Solution:
Composition per liter:
MgSO 4 ·7H 2 O............................................................................24.65g
NaCl............................................................................................23.4g
Tri-sodium EDTA.......................................................................4.53g
CaCl 2 ·2H 2 O ................................................................................2.94g
FeSO 4 ·7H 2 O................................................................................ 1.11g
MnSO 4 ·4H 2 O.........................................................................112.0mg
H 3 BO 3 ......................................................................................31.0mg
ZnSO 4 ·7H 2 O............................................................................29.0mg
Co(NO 3 ) 2 ·6H 2 O.......................................................................29.0mg
CuSO 4 ·5H 2 O............................................................................25.4mg
Na 2 MoO 4 ·4H 2 O.......................................................................24.0mg
Preparation of Basal Salts Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Phosphate Solution:
Composition per liter:
KH 2 PO 4 .......................................................................................75.0g
K 2 HPO 4 .......................................................................................78.0g
Preparation of Phosphate Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution
Composition per 100.0mL:
Tri-sodium EDTA..................................................................200.0mg
Nicotinic acid.........................................................................100.0mg
Thiamine................................................................................100.0mg
p-Aminobenzoic acid...............................................................50.0mg
Calcium pantothenate ..............................................................50.0mg
Pyridoxine hydrochloride ........................................................50.0mg
Folic acid..................................................................................50.0mg
Biotin .........................................................................................5.0mg
Vitamin B 12 ................................................................................1.0mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 999.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C. Aseptically add 1.0mL sterile vitamin solution. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Porphyrobacter tepidarius, Porphyrobacter
cryptus, and Porphyrobacter sp.