Potato Carrot Agar with Manganese

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Carrot Agar with Manganese

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Potatoes.......................................................................................20.0g
Carrots.........................................................................................20.0g
Manganese solution .................................................................10.0mL
pH 6.5 ± 0.2 at 25°C
Manganese Solution:
Composition per 100.0mL :
MnCl 2 ·4H 2 O................................................................................15.0g
Preparation of Manganese Solution: Add MnCl 2 ·4H 2 O to to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Wash and peel potatoes and carrots.
Grate potatoes and carrots and place in 1.0L of tap water. Gently heat
and bring to boiling. Boil for 30 min. Filter through cheesecloth. Bring
volume of filtrate to 900.0mL with tap water. Add 20.0g of agar. Gently
heat and bring to boiling. Distribute into flasks. Autoclave for 20 min
at 15 psi pressure–121°C. Cool to 55°C. Aseptically add 100.0mL of
sterile manganese solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into tubes.
Use:  For the selective cultivation and maintenance of Alternaria, Epic-
occum, and Phoma species. The additon of manganese inhibits the
growh of other fungi.