Potato Dextrose Agar (PDA Agar)

[所属分类:培养基配方] [发布时间:2021-7-12] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Dextrose Agar
(PDA Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................20.0g
Agar............................................................................................15.0g
Potatoes, infusion from.................................................................1.0L
Potatoes, Infusion From:
Composition per liter:
Potatoes.....................................................................................300.0g
Preparation of Potatoes, Infusion From:  Peel and dice potatoes.
Add 500.0mL of distilled/deionized water. Gently heat and bring to
boiling. Continue boiling for 30 min. Filter through cheesecloth. Bring
volume of filtrate to 1.0L.
Preparation of Medium: To 1.0L of potato infusion, add glucose
and agar. Mix thoroughly. Gently heat and bring to boiling. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Bacillus megaterium,
Bacillus subtilis, Pseudomonas lindbergii, Pseudomonas syringae,
Streptomyces testaceus, and Xanthomonas campestris.