Potato Dextrose Agar, 1/3 Strength (ATCC Medium 2369)

[所属分类:培养基配方] [发布时间:2021-7-12] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Dextrose Agar, 1/3 Strength
(ATCC Medium 2369)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................6.67g
Agar ............................................................................................15.0g
Potatoes, infusion from..........................................................333.0mL
pH 5.6 ± 0.2 at 25°C
Potato Infusion:
Composition  per liter:
Potatoes.....................................................................................300.0g
Preparation of Potato Infusion:  Peel and dice potatoes. Add
500.0mL of distilled/deionized water. Gently heat and bring to boiling.
Continue boiling for 30 min. Filter through cheesecloth. Bring volume
to 1.0L. Reserve filtrate.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of yeasts and molds.