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Potato Dextrose Agar and Yeast Medium (PDA and Yeast Medium) (ATCC Medium 104)
[所属分类:培养基配方] [发布时间:2021-7-12] [发布人:网站管理员2] [阅读次数:] [返回]
Potato Dextrose Agar and Yeast Medium
(PDA and Yeast Medium)
(ATCC Medium 104)
Agar............................................................................................15.0g
KH 2 PO 4 .........................................................................................2.0g
Yeast extract..................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................0.5g
Potato infusion.......................................................................500.0mL
Potato Infusion:
Composition per 500.0mL:
Potatoes.....................................................................................300.0g
Preparation of Potato Infusion: Peel and dice potatoes. Add
500.0mL of distilled/deionized water. Gently heat and bring to boiling.
Continue boiling for 30 min. Filter through cheesecloth. Reserve fil-
trate.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Pseudomonas fluore-
scens, Streptoverticillium baldaccii, and Xanthomonas campestris.
(PDA and Yeast Medium)
(ATCC Medium 104)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Glucose.......................................................................................20.0gAgar............................................................................................15.0g
KH 2 PO 4 .........................................................................................2.0g
Yeast extract..................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................0.5g
Potato infusion.......................................................................500.0mL
Potato Infusion:
Composition per 500.0mL:
Potatoes.....................................................................................300.0g
Preparation of Potato Infusion: Peel and dice potatoes. Add
500.0mL of distilled/deionized water. Gently heat and bring to boiling.
Continue boiling for 30 min. Filter through cheesecloth. Reserve fil-
trate.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Pseudomonas fluore-
scens, Streptoverticillium baldaccii, and Xanthomonas campestris.
