Potato Sucrose Agar

[所属分类:培养基配方] [发布时间:2021-7-12] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Sucrose Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Potato extract............................................................................200.0g
Sucrose........................................................................................20.0g
Agar............................................................................................20.0g
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Wash and peel potatoes. Dice potatoes
and place in a muslin bag. Suspend bag in 500.0mL of tap water. Gen-
tly heat and bring to boiling. Boil for 10 min. Remove the muslin bag
with the potatoes. Bring volume of potato extract to 1.0L with tap wa-
ter. Add 20.0g of agar and 5.0g of malt extract. Mix thoroughly. Adjust
pH to 6.5 with CaCO 3 . Gently heat and bring to boiling. Distribute into
tubes or flasks. Autoclave for 20 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Candida famata, Colletotri-
chum capsici, Colletotrichum coccodes, Colletotrichum crassipes, Col-
letotrichum dematium, Colletotrichum gloesporioides, Microdochium
nivale, and numerous Fusarium species.