Powell and Errington’s Medium

[所属分类:培养基配方] [发布时间:2021-7-13] [发布人:网站管理员2] [阅读次数:] [返回]

Powell and Errington’s Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1060.0mL:

Solution 1.................................................................................50.0mL
Solution 3.................................................................................50.0mL
Solution 4.................................................................................10.0mL
Solution 2...................................................................................5.0mL
pH 7.0 ± 0.2 at 25°C
Solution 1:
Composition per liter:
(NH 4 ) 2 HPO 4 ..............................................................................238.0g
K 2 SO 4 ..........................................................................................70.0g
NaH 2 PO 4 ·2H 2 O...........................................................................31.0g
Preparation of Solution 1: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly.
Solution 2:
Composition per liter:
MgO............................................................................................10.0g
FeCl 3 ·6H 2 O...................................................................................5.4g
CaCO 3 ...........................................................................................2.0g
ZnSO 4 ·7H 2 O...............................................................................1.44g
MnSO 4 ·4H 2 O..............................................................................1.11g
Na 2 MoO 4 ·2H 2 O ..........................................................................0.49g
CoSO 4 ·7H 2 O...............................................................................0.28g
CuSO 4 ·5H 2 O...............................................................................0.25g
H 3 BO 4 .......................................................................................0.062g
HCl, concentrated ....................................................................50.0mL
Preparation of Solution 2: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly.
Solution 3:
Composition per 50.0mL:
Citric acid......................................................................................4.2g
Glucose .........................................................................................3.6g
L- Glutamic acid...........................................................................2.94g
Succinic acid...............................................................................1.18g
Preparation of Solution 3: Add components to distilled/deionized
water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.
Solution 4:
Composition per 10.0mL:
Na 2 S 2 O 3 ·5H 2 O............................................................................1.24g
Preparation of Solution 4: Add Na 2 S 2 O 3 ·5H 2 O to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add 50.0mL of solution 1 and 5.0mL of
solution 2. Mix thoroughly. Bring volume to 1.0L with distilled/deion-
ized water. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C. Adjust pH to 7.0 with sterile NaOH. Aseptically add 50.0mL of
solution 3 and 10.0mL of sterile solution 4. Mix thoroughly. Aseptical-
ly distribute into sterile tubes or flasks.
Use: For the cultivation of a variety of heterotrophic microorganisms.