PP Medium

[所属分类:培养基配方] [发布时间:2021-7-13] [发布人:网站管理员2] [阅读次数:] [返回]

PP Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone.........................................................................10.0g
Pancreatic digest of peptone.......................................................10.0g
Ribonucleic acid from Torula yeast..............................................1.0g
Asolectin.......................................................................................0.2g
Artificial seawater..................................................................167.0mL
Vitamin solution.........................................................................2.0mL
Artificial Seawater:
Composition per 167.0mL:
Aqua-Marin sea salts ..................................................................6.95g
Source:  Aqua-Marin sea salts are available from Aquatrol, Inc., Ana-
heim, CA.
Preparation of Artificial Seawater: Add Aqua-Marin sea salts to
distilled/deionized water and bring volume to 167.0mL Mix thorough-
ly. Filter sterilize.
Vitamin Solution:
Composition per 100.0mL:
Thiamine·HCl ........................................................................150.0mg
Calcium  D -(+)-pantothenate ..................................................100.0mg
Folic acid .................................................................................50.0mg
Nicotinamide............................................................................50.0mg
Pyridoxal·HCl..........................................................................50.0mg
Riboflavin................................................................................50.0mg
DL -6 Thioctic acid......................................................................1.0mg
Biotin solution .........................................................................10.0mL
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize. For long-term storage, preserve under nitrogen at −20°C.
Biotin Solution:
Composition per 10.0mL:
Biotin .......................................................................................0.01mg
Preparation of Biotin Solution: Add biotin to 10.0mL of absolute
ethanol. Mix thoroughly.
Preparation of Medium: Add ascolectin to 500.0mL of distilled/
deionized water. Gently heat to 80°C. Mix thoroughly. Add other com-
ponents, except artificial seawater and vitamin solution, to distilled/de-
ionized water and bring volume to 831.0mL. Mix thoroughly. Adjust
pH to 7.2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically
add 167.0mL of sterile artificial seawater and 2.0mL of sterile vitamin
solution. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation of Potomacus pottsi.