PPGA Medium

[所属分类:培养基配方] [发布时间:2021-7-13] [发布人:网站管理员2] [阅读次数:] [返回]

PPGA Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................18.0g
Glucose .........................................................................................5.0g
Peptone..........................................................................................5.0g
NaCl..............................................................................................3.0g
Na 2 HPO 4 .......................................................................................1.2g
KH 2 PO 4 .........................................................................................0.5g
Potato decoction............................................................................1.0L
pH 7.0 ± 0.2 at 25°C
Potato Decoction:
Composition per liter:
Potatoes.....................................................................................200.0g
Preparation of Potato Decoction: Peel and dice potatoes. Add
1.0L of distilled/deionized water. Gently heat and bring to boiling.
Continue boiling for 20 min. Filter through two layers of cheesecloth.
Bring volume of filtrate to 1.0L with distilled/deionized water.
Preparation of Medium: Combine components. Gently heat and
bring to boiling. Adjust pH to 7.0. Distribute into tubes or flasks. Au-
toclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri
dishes or leave in tubes.
Use: For the cultivation of Burkholderia glumae and Burkholderia
plantarii.