PPLO Agar

[所属分类:培养基配方] [发布时间:2021-7-13] [发布人:网站管理员2] [阅读次数:] [返回]

PPLO Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, infusion from............................................................50.0g
Agar ............................................................................................14.0g
Peptone........................................................................................10.0g
NaCl..............................................................................................5.0g
Bovine serum.........................................................................100.0mL
pH 7.8 ± 0.2 at 25°C
Preparation of Medium: Add components, except bovine serum,
to distilled/deionized water and bring volume to 900.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile bovine se-
rum. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the isolation and cultivation of Mycoplasma species (pleuro-
pneumonia-like organisms).