PPLO Broth, pH 7.6 with Additives for Mycoplasma

[所属分类:培养基配方] [发布时间:2021-7-13] [发布人:网站管理员2] [阅读次数:] [返回]

PPLO Broth, pH 7.6 with Additives for Mycoplasma

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

L- Cysteine·HCl·H 2 O......................................................................1.0g
PPLO broth............................................................................700.0mL
Horse serum, not inactivated .................................................200.0mL
Yeast extract solution, fresh...................................................100.0mL
pH 7.6 ± 0.2 at 25°C
PPLO Broth:
Composition per liter:
Beef heart, infusion from............................................................50.0g
Peptone .......................................................................................10.0g
NaCl..............................................................................................5.0g
Preparation of PPLO Broth: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and
bring to boiling.
Yeast Extract Solution:
Composition per 300.0mL:
Baker’s yeast, live, pressed, starch-free.....................................75.0g
Preparation of Yeast Extract Solution : Add the live Baker’s
yeast to 300.0mL of distilled/deionized water. Autoclave for 90 min at
15 psi pressure–121°C. Allow to stand. Remove supernatant solution.
Adjust pH to 6.6–6.8. Filter sterilize.
Preparation of Medium: Add components, except fresh yeast ex-
tract solution and horse serum, to distilled/deionized water and bring vol-
ume to 700.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asep-
tically add sterile fresh yeast extract solution and horse serum. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Mycoplasma faucium.