PPLO Broth with Bovine Serum

[所属分类:培养基配方] [发布时间:2021-7-13] [发布人:网站管理员2] [阅读次数:] [返回]

PPLO Broth with Bovine Serum

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, infusion from............................................................50.0g
Peptone........................................................................................10.0g
NaCl..............................................................................................5.0g
Phenol Red (1% solution)..........................................................2.0mL
Yeast extract solution, fresh...................................................100.0mL
Glucose solution ......................................................................25.0mL
Bovine serum, filter sterilized..................................................10.0mL
pH 7.5 ± 0.2 at 25°C
Yeast Extract Solution:
Composition per 300.0mL:
Baker’s yeast, live, pressed, starch-free......................................75.0g
Preparation of Yeast Extract Solution : Add the live Baker’s
yeast to 300.0mL of distilled/deionized water. Autoclave for 90 min at
15 psi pressure–121°C. Allow to stand. Remove supernatant solution.
Adjust pH to 6.6–6.8. Filter sterilize.
Glucose Solution:
Composition per 100.0mL:
D -Glucose....................................................................................20.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components—except fresh yeast ex-
tract solution, glucose solution, and bovine serum—to distilled/deion-
ized water and bring volume to 865.0mL. Mix thoroughly. Gently heat
and bring to boiling. Adjust pH to 7.5. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile fresh yeast
extract solution, glucose solution, and bovine serum. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Acholeplasma morum.