PPLO Broth without Crystal Violet

[所属分类:培养基配方] [发布时间:2021-7-13] [发布人:网站管理员2] [阅读次数:] [返回]

PPLO Broth without Crystal Violet

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, infusion from............................................................50.0g
Peptone .......................................................................................10.0g
NaCl..............................................................................................5.0g
Thallium acetate (optional)...........................................................0.5g
Penicillin (optional)............................................................. 100,000U
Ascitic fluid ...........................................................................250.0mL
pH 7.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except ascitic fluid, to
distilled/deionized water and bring volume to 750.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to less than
37°C. Aseptically add sterile ascitic fluid. If desired, 0.5g of thallium
acetate or 100,000U of penicillin may be added for a more selective
medium. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the enrichment of pleuro-pneumonia-like organisms
(PPLOs) and Mycoplasma species from clinical specimens.