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PPLO Broth without Crystal Violet with Sodium Acetate, Fresh Yeast Extract, and Calf Serum (ATCC Medium 843)
[所属分类:培养基配方] [发布时间:2021-7-13] [发布人:网站管理员2] [阅读次数:] [返回]
PPLO Broth without Crystal Violet with Sodium
Acetate, Fresh Yeast Extract, and Calf Serum
(ATCC Medium 843)
Peptone .......................................................................................10.0g
NaCl..............................................................................................5.0g
Sodium acetate..............................................................................1.0g
Calf serum..............................................................................100.0mL
Yeast extract solution, fresh.....................................................50.0mL
pH 7.8 ± 0.2 at 25°C
Yeast Extract Solution:
Composition per 300.0mL:
Baker’s yeast, live, pressed, starch-free......................................75.0g
Preparation of Yeast Extract Solution : Add the live Baker’s
yeast to 300.0mL of distilled/deionized water. Autoclave for 90 min at
15 psi pressure–121°C. Allow to stand. Remove supernatant solution.
Adjust pH to 6.6–6.8. Filter sterilize.
Preparation of Medium: Add components, except fresh yeast ex-
tract solution and calf serum, to distilled/deionized water and bring
volume to 850.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 25°C. Aseptically add sterile fresh yeast ex-
tract solution and calf serum. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation and maintenance of Acholeplasma laidlawii.
Acetate, Fresh Yeast Extract, and Calf Serum
(ATCC Medium 843)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Beef heart, infusion from............................................................50.0gPeptone .......................................................................................10.0g
NaCl..............................................................................................5.0g
Sodium acetate..............................................................................1.0g
Calf serum..............................................................................100.0mL
Yeast extract solution, fresh.....................................................50.0mL
pH 7.8 ± 0.2 at 25°C
Yeast Extract Solution:
Composition per 300.0mL:
Baker’s yeast, live, pressed, starch-free......................................75.0g
Preparation of Yeast Extract Solution : Add the live Baker’s
yeast to 300.0mL of distilled/deionized water. Autoclave for 90 min at
15 psi pressure–121°C. Allow to stand. Remove supernatant solution.
Adjust pH to 6.6–6.8. Filter sterilize.
Preparation of Medium: Add components, except fresh yeast ex-
tract solution and calf serum, to distilled/deionized water and bring
volume to 850.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 25°C. Aseptically add sterile fresh yeast ex-
tract solution and calf serum. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation and maintenance of Acholeplasma laidlawii.
