PPYG Medium

[所属分类:培养基配方] [发布时间:2021-7-13] [发布人:网站管理员2] [阅读次数:] [返回]

PPYG Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Glucose.........................................................................................5.0g
Peptone .........................................................................................5.0g
NaCl..............................................................................................1.5g
Na 2 HPO 4 ·12H 2 O...........................................................................1.5g
Yeast extract..................................................................................1.5g
MgCl 2 ·6H 2 O .................................................................................0.1g
Na 2 CO 3 solution ......................................................................50.0mL
Glucose solution......................................................................50.0mL
pH 10.5–11.0 at 25°C
Na 2 CO 3 Solution:
Composition  per 50.0mL:
Na 2 CO 3  .......................................................................................5.03g
Preparation of Na 2 CO 3 Solution:  Add Na 2 CO 3  to distilled/de-
ionized water and bring volume to 50.0mL. Mix thoroughly. Filter ster-
ilize.
Glucose Solution:
Composition per 50.0mL:
D -Glucose......................................................................................5.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 50.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add components, except Na 2 CO 3  solu-
tion and glucose solution, to distilled/deionized water and bring vol-
ume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile Na 2 CO 3  solution and glucose solution. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Exiguobacterium auran-
tiacum.