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Pre-Enrichment HiVeg Broth Base with Magnesium Sulfate and Calcium Chloride

[所属分类:培养基配方] [发布时间:2021-7-13] [发布人:网站管理员2] [阅读次数:] [返回]

Pre-Enrichment HiVeg Broth Base
with Magnesium Sulfate and Calcium Chloride

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................20.0g
Plant special peptone ..................................................................10.0g
Na 2 HPO 4 .......................................................................................7.1g
KCl................................................................................................1.0g
NaCl..............................................................................................1.0g
Magnesium sulfate solution.....................................................10.0mL
Calcium chloride soltuion........................................................10.0mL
pH 8.3 ± 0.2 at 25°C
Source: This medium, without magnesium sulfate and calcium chlo-
ride solutions, is available as a premixed powder from HiMedia.
Magnesium Sulfate Solution:
Composition per 10.0mL:
MgSO 4 ........................................................................................0.01g
Preparation of Magnesium Sulfate Solution: Add MgSO 4 to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Calcium Chloride Solution:
Composition per 10.0mL:
CaCl 2 ...........................................................................................0.01g
Preparation of Calcium Chloride Solution: Add CaCl 2 to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except magnesium
chloride solution and calcium chloride solution, to distilled/deionized
water and bring volume to 980.0mL. Mix thoroughly. Gently heat
while stirring and bring to boiling. Distribute into tubes or flasks. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asep-
tically add 10.0mL of sterile magnesium sulfate solution and 10.0mL
of sterile calcium chloride solution. Mix thoroughly. Aseptically dis-
tribute to sterile tubes or flasks.
Use: For the isolation, enrichment, and cultivation of Yersinia entero-
colitica from foods.

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Pre-Enrichment HiVeg Broth Base with Magnesium Sulfate and Calcium Chloride