Presence-Absence Broth (P-A Broth)

[所属分类:培养基配方] [发布时间:2021-7-13] [发布人:网站管理员2] [阅读次数:] [返回]

Presence-Absence Broth
(P-A Broth)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................10.0g
Lactose..........................................................................................7.5g
Pancreatic digest of gelatin...........................................................5.0g
Beef extract...................................................................................3.0g
NaCl..............................................................................................2.5g
K 2 HPO 4 .....................................................................................1.375g

KH 2 PO 4 .....................................................................................1.375g

Sodium lauryl sulfate..................................................................0.05g
Bromcresol Purple .....................................................................8.5mg
pH 6.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 333.0mL. Mix thoroughly. Distribute into
screw-capped 250.0mL milk dilution bottles in 50.0mL volumes. Au-
toclave for 15 min at 15 psi pressure–121°C.
Use: For the detection of coliform bacteria in water from treatment
plants or distribution systems using the presence-absence coliform test.