Preston Enrichment Broth

[所属分类:培养基配方] [发布时间:2021-7-14] [发布人:网站管理员2] [阅读次数:] [返回]

Preston Enrichment Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef extract.................................................................................10.0g
Peptone........................................................................................10.0g
NaCl..............................................................................................5.0g
Horse blood, lysed ...................................................................50.0mL
Antibiotic solution ...................................................................10.0mL
pH 7.5 ± 0.2 at 25°C
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide..............................................................................0.1g
Rifampicin ..................................................................................0.01g
Trimethoprim lactate...................................................................0.01g
Polymyxin B.............................................................................5000U
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except horse blood and
antibiotic solution, to distilled/deionized water and bring volume to
940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile horse blood and antibiotic solution. Mix thoroughly. Asep-
tically distribute into sterile tubes or flasks.
Use: For the isolation and enrichment of Campylobacter species from
foods.