Prey Seawater Broth (DSMZ Medium 1013)

[所属分类:培养基配方] [发布时间:2021-7-14] [发布人:网站管理员2] [阅读次数:] [返回]

Prey Seawater Broth
(DSMZ Medium 1013)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Artificial seawater..................................................................700.0mL
Trace elements solution...........................................................10.0mL
pH 7.7 ± 0.3 at 25°C
Artificial Seawater:
Composition per liter:
NaCl............................................................................................27.7g
MgSO 4 ·7H 2 O................................................................................7.0g
MgCl 2 ·6H 2 O..................................................................................5.5g
CaCl 2 ·2H 2 O.................................................................................2.25g
KCl..............................................................................................0.65g
NaBr..............................................................................................0.1g
H 3 BO 3 ......................................................................................30.0mg
SrCl 2 ·6H 2 O...............................................................................15.0mg
Citric acid.................................................................................10.0mg
KI.............................................................................................0.05mg
Preparation of Artificial Seawater: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7H 2 O............................................................................... 3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O............................................................................... 0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O...................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
KAI(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
CuSO 4 ·5H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.01g
NiCl 2 ·6H 2 O...............................................................................0.025g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add remaining components. Add distilled/deionized water to
1.0L.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.7. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Grow prey
bacterium E. coli DSM 15416) on agar plates using marine agar 2216.
For 50mL prey seawater broth use one 24 h old agar plate and wash off
grown cells in 3.0–5.0mL artificial seawater. The prey seawater broth
should contain approx. 10 7 to 10 9 cells. Inoculate the medium immedi-
ately after preparation and incubate the suspension with shaking until
a decrease in turbidity is visible.
Use:  For the cultivation of Bacteriovorax litoralis.