Propionibacterium Medium

[所属分类:培养基配方] [发布时间:2021-7-14] [发布人:网站管理员2] [阅读次数:] [返回]

Propionibacterium Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................10.0g
Na 2 HPO 4 ·2H 2 O.............................................................................3.0g
KH 2 PO 4 .........................................................................................1.0g
Lactate solution........................................................................40.0mL
pH 7.0 ± 0.2 at 25°C
Lactate Solution:
Composition per 100.0mL:
Sodium lactate.............................................................................70.0g
Preparation of Lactate Solution:  Add 70.0g of sodium lactate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Preparation of Medium: Add components, except lactate solution,
to distilled/deionized water and bring volume to 960.0mL. Mix thor-
oughly. Add 40.0mL of lactate solution. Mix thoroughly. Adjust pH to
7.0. Distribute into screw-capped tubes. Autoclave for 15 min at 15 psi
pressure–121°C.
Use:  For the cultivation of Propionibacterium acidipropionici, Propi-
onibacterium freudenreichii, Propionibacterium jensenii, and Propioni-
bacterium thoenii.