Propionispora Medium (DSMZ Medium 503c)

[所属分类:培养基配方] [发布时间:2021-7-14] [发布人:网站管理员2] [阅读次数:] [返回]

Propionispora Medium
(DSMZ Medium 503c)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Solution A..............................................................................940.0mL
Solution B................................................................................50.0mL
Solution C................................................................................10.0mL
Solution D................................................................................10.0mL
pH 7.1 ± 0.2 at 25°C
Solution A:
Composition per 940.0mL:
Yeast extract..................................................................................1.0g
NaCl..............................................................................................1.0g
KCl................................................................................................0.5g
MgCl 2 ·6H 2 O .................................................................................0.4g
KH 2 PO 4 .........................................................................................0.2g
NH 4 Cl .........................................................................................0.25g
CaCl 2 ·2H 2 O ................................................................................0.15g
Resazurin ...................................................................................0.5mg
Trace elements solution SL-10..................................................1.0mL
Preparation of Solution A: Prepare under 80% N 2 + 20% CO 2 gas
atmosphere. Add components to distilled/deionized water and bring vol-
ume to 940.0mL. Mix thoroughly. Adjust pH to 7.2. Sparge with 80% N 2
+ 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C.
Trace Elements Solution SL-10:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O...........................................................................190.0mg
MnCl 2 ·4H 2 O ..........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O.......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
H 3 BO 3 ........................................................................................6.0mg
CuCl 2 ·2H 2 O...............................................................................2.0mg
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly. Sparge with 80% N 2 + 20% CO 2 . Autoclave for 15 min at 15
psi pressure–121°C.
Solution B:
Composition per 100.0mL:
NaHCO 3 ........................................................................................5.0g
Preparation of Solution B:  Add NaHCO 3 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Sparge with
100% N 2 gas mixture. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 25°C.
Solution C:
Composition per 100.0mL:
Fructose.........................................................................................5.0g
Preparation of Solution C:  Add fructose to distilled/deionized wa-
ter and bring volume to 100.0mL. Mix thoroughly. Sparge with 100%
N 2 gas mixture. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 25°C.
Solution D :
Composition per 10.0mL:
Na 2 S·9H 2 O................................................................................0.125g
Preparation of Solution D: Add Na 2 S·9H 2 O to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Autoclave under
100% N 2 for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Prepare and dispense medium under 80%
N 2 + 20% CO 2 gas atmosphere. Sequentially add 50.0mL solution B,
10.0mL solution C, and 10.0mL solution D, to 940.0mL solution A. Dis-
tribute anaerobically under 80% N 2  + 20% CO 2 into appropriate ves-
sels. The pH should be 7.0–7.2.
Use: For the cultivation of Propionispora vibrioides.