Proskauer-Beck Medium for Mycobacterium

[所属分类:培养基配方] [发布时间:2021-7-14] [发布人:网站管理员2] [阅读次数:] [返回]

Proskauer-Beck Medium for Mycobacterium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Asparagine....................................................................................5.0g
KH 2 PO 4 .........................................................................................5.0g
Magnesium citrate ........................................................................2.5g
MgSO 4 ·7H 2 O................................................................................0.6g
Glycerol ...................................................................................20.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, one at a time, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Make sure one salt is totally dissolved before the next one is added. Ad-
just pH to 7.8 with 40% NaOH. Autoclave for 15 min at 15 psi pres-
sure–121°C. The pH of the medium after autoclaving should be 7.4.
Filter through Whatman #1 filter paper to remove any precipitate. Dis-
tribute into tubes or flasks. Autoclave again for 15 min at 15 psi pres-
sure–121°C.
Use:  For the cultivation and maintenance of Mycobacterium tubercu-
losis.